English translation for "collagen type i"
|
- 胶原Ⅰ型
Related Translations:
- Example Sentences:
| 1. | Cell adhesion dramatically increased after 6h by fibronectin and after 12h by collagen type i in monolayer culture
1型细胞为多角形或长校形， actin表达较少并相对聚集； 11型细胞细胞圆形，铺展良好， 。 | | 2. | Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells . only after adhering to surface followed by spreading can cells develop and proliferate . most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity , which may correlates to the low degree of cell attachment . modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation . cell attachment to substrate is primarily mediated by integrins , a widely expressed family of heterodimeric surface receptors . most extrcellular matrix proteins such as fibronectin , osteopontin , collagen type i , bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin . the main aim of this research is to measure , assess adhesion , proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin , collagen type i or biotie gen , which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture . ( 2 ) adhesion , proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed . ( 3 ) also , adhesion , proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin , or collagen type i respectively . ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo . this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities
因此将这些蛋白包被、固定到材料表面，观察骨组织工程种子细胞mscs细胞的粘附、生长特性是本研究的中心环节，并从以下方面进行探讨： ( 1 )采用不同原代细胞分离方法，研究其对mscs细胞的生物学特性影响。 ( 2 )检测基因胜肽胶对mscs细胞粘附、增殖及分化的影响。 ( 3 )分别采用型胶原及纤维粘连蛋白( fibronectin ， fn )包被聚乙醇酸-乳酸共聚物( poly ( 1actide - co - glycolide ) ， plga )膜及多孔块型plga材料，观察细胞在单层或三维培养状态下，型胶原及fn对mscs细胞粘附、增殖及向成骨细胞分化效应及能力。 | | 3. | There was no difference in other biologic characteristic of mscs between the two separation method , such as cell anchorage ratio and clone formation ratio . ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ) . big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate , around the big aperture there were many round micropores about 5 m size . all of the structure were equal and uniform , which satisfied the further research work . ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h , cell number was ( 1 . 5 0 . 18 ) 105 in the plga film coated with biotiegen group , which was significantly higher than that in plga film group ( p < 0 . 01 ) and higher than that in coverslip group ( p < 0 . 05 ) , which cell number was ( 1 . 04 0 . 21 ) 105 . after 6h and 12h biotiegen could not promote cell adhesion , and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days . ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型胶原、纤维粘连蛋白促进细胞增殖，细胞接种后3 、 6 、 gd三个检测时间点，实验组细胞均明显高于对照组。与1型胶原相比，纤维粘连蛋白刺激作用更强。 ） i型胶原、纤维粘连蛋白尚能诱导mscs细胞向成骨细胞分化，不仅表达成骨细胞标志物ocn 、 alp 、 opnmrna ，而且碱性磷酸酶活性明显增高，碱性磷酸酶及钙结节7第四军医大学博士学位论文一染色均强阳性， i型胶原组mscs细胞碱性磷酸酶活性较fn组更高，有显著性差异；同时，兔疫组化染色表明，经纤维粘连蛋白作用的mscs1型胶原表达阳性。 |
- Similar Words：
- "collagen stroma" English translation, "collagen sugar" English translation, "collagen suture" English translation, "collagen thesaurismosis" English translation, "collagen tissue" English translation, "collagen type ii" English translation, "collagen type iii" English translation, "collagen type iv" English translation, "collagen vascular disease" English translation, "collagen volume fraction" English translation
|
|
|
